产品货号:
Z37606
中文名称:
GAPDHS Purified抗体
英文名称:
Anti-GAPDHS Purified Antibody (Clone#Hs-8)
产品规格:
0.1mg(C100)
发货周期:
1~3天
产品价格:
询价
抗体名称 | Anti-GAPDHS Purified Antibody (Clone#Hs-8) |
指标别名 | GAPD2;GAPDH 2;GAPDH2;GAPDHS;GAPDS;HSD 35 |
克隆性 | Monoclonal |
检验物种 | pig,human |
应用范围 | WB,ICC,FCM |
基因名称 | GAPDHS |
克隆号 | Hs-8 |
抗体来源 | Mouse |
抗体类型 | IgM |
免疫原 | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol,30mM benzaminidine.The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.The antibody Hs-8 reacts with GAPDHS,the sperm-specific glyceraldehyde phosphate dehydrogenase,which is an intra-acrosomal protein. |
计算分子量 | 45kDa |
成分 | Tris buffered saline (TBS),pH8.0,15mM sodium azide |
纯化方式 | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
浓度 | 1mg/mL |
产品形态 | 溶液 |
保存条件 | Store at 2~8℃.Do not freeze. |
背景资料 | GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase,also known as GAPD2,GAPDS,HSD-35,or GAPDH-2,is a glycolytic enzyme that plays an important role in carbohydrate metabolism.Like its somatic cell counterpart,this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate.During spermiogenesis,this enzyme may play an important role in regulating the switch between different energy-producing pathways,and it is required for sperm motility and male fertility.It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction. |
Uniprot ID | O14556 |
基因名全称 | Glyceraldehyde-3-phosphate dehydrogenase,spermato |
蛋白名全称 | Glyceraldehyde-3-phosphate dehydrogenase,testis-specific |
推荐稀释比 | Immunocytochemistry:10μg/mL;membrane permeabilization (acetone) is essential. Flow cytometry:3~12μg/mL.Intraacrosomal staining. |
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